Schedule
| Week 1 | |||||||
| Monday 31.10 | Tuesday 01.11 | Wednesday 02.11 | Thursday 03.11 | Friday 04.11 | Saturday 05.11 | Sunday 06.11 | |
| Morning | FNIH course (theory only): Introduction to Genome editing and gene drives | FNIH course (theory only): Introduction to Genome editing and gene drives |
Introduction to the course, team-building, Introduction to molecular biology, in silico cloning |
In silico cloning, sgRNA design, online tools | Molecular biology |
sgRNA vector cloning, In vitro cutting of plasmid DNA with Cas9 protein |
Free Day – Excursion |
| Lunch | |||||||
| Afternoon | FNIH course (theory only): Introduction to Genome editing and gene drives | FNIH course (theory only): Introduction to Genome editing and gene drives | Introduction to practical aspects of genome editing tools and gene drives | Molecular biology Work on student’s own target gene |
sgRNA vector cloning | Gibson Assembly and Golden Gate cloning |
| Week 2 | |||||||
| Monday 07.11 | Tuesday 08.11 | Wednesday 09.11 | Thursday 10.11 | Friday 11.11 | Saturday 12.11 | ||
| Morning |
Wrap-up of week 1 Introduction to zebrafish, microinjections |
Introduction to zebrafish microinjections | Introduction to Drosophila | Introduction to mosquito | Student presentations and Wrap-up | Departure | |
| Lunch | |||||||
| Afternoon | Cas9 protein purification or Drosophila cell culture | Cas9 protein purification or Drosophila cell culture |
Building your own microinjector | Building your own microinjector | Student presentations and Wrap-up |